Berberine exposure triggers developmental effects on. Dugesia japonica is a typical planarian species that is widely. Mortality and antioxidant responses in the planarian. Draft genome of dugesia japonica provides insights into. The flatworm planarian dugesia japonica inhabits fresh water in east asian areas and is the most common planarian in japan. It has been reported from sicily, 1 elba and mallorca, 2 eivissa, 3. Unusually large number of mutations in asexually reproducing.
Sep 22, 2016 the planarian flatworm is an emerging model that is useful for studying homeostasis and regeneration due to its unique adult stem cells ascs. Comparative transcriptome analysis between planarian. Molecular cloning and expression pattern of the djstag. These active specimens are large and brown in color. Here, we develop a pharmacological method to target ion transporters, uncovering a role for membrane voltage as a key regulator of anterior polarity in regenerating planaria. Comparative transcriptome analysis between planarian dugesia. Dugesia japonica is a typical planarian species that is. Draft genome of dugesia japonica provides insights into conserved. Comparative transcriptome analysis between planarian dugesia japonica to construct a database of the headregion transcriptome, and then performed comparative analyses. Background the freshwater planarian dugesia japonica d. The importance of immune system prompted us to search for immunerelated genes and rigilike receptor. Role of mechanics for development and regeneration starting from a uniform system, how do spatial patterns get established. Fgfr1 fibroblast growth factor receptor 1 precursor.
Here, we use the freshwater planarian dugesia japonica. Previously, planaria were found to share mammalian. Title genome analysis of the planarian dugesia japonica. In addition to the four genomes presented here, two other complete or nearcomplete triclad genomes have been published. Dugesia sicula is a species of dugesiid triclad that lives in freshwater bodies of the mediterranean basin, where it is widely distributed. By using 16s rrna sequencing of dna and rna, culturebased approaches, and wholegenome sequencing, we characterized the diversity of the d. Both approaches can produce visually striking phenotypes at high penetrance for example, regeneration of bipolar animals 1621. Mechanics dictate where and how freshwater planarians.
Dugesia japonica is the best suited of three planarian species for highthroughput toxicology screening danielle ireland a, veronica bochenek a, daniel chaiken b, christina rabeler a, sumi onoe b, ameet. Deep sequencing identifies regulated small rnas in dugesia. Dugesia japonica, a freshwater planarian, can regenerate a complete individual from a head, trunk or tail fragment via activation of somatic pluripotent stem cells named neoblasts. The planarian flatworm is an emerging model that is useful for studying homeostasis and regeneration due to its unique adult stem cells ascs. The file may contain a single sequence or a list of sequences. Several planarian species, such as dugesia japonica and schmidtea mediterranea, can. The plant database covers the type of plant, its size and its most suitable environment. Rnai of cnsexpressed gene djslk induces morphogenetic. A central nervous system in wildtype dugesia japonica with wholemount in situ hybridization of pc2. Nov 25, 2015 a multinational team of scientists led by tufts university biologists has succeeded in inducing a species of planarian, girardia dorotocephala, to grow heads and brains characteristic of other. Phylogenetic analysis shows that djpreb is prebpreblike members.
Dugesia is the type genus of the family dugesiidae. A chemical genetics approach reveals h,katpasemediated. Characterization and expression of djpreb gene in the. Media in category dugesia japonica the following 2 files are in this category, out of 2 total. A clonal asexual strain ssp of dugesia japonica was used for all experiments asami et al. Manage plant information in as much detail as you like including. Platanus is known to be a powerful assembly software for highly. Planarians exhibit traits of cephalization but are unique among bilaterians in that they ingest food by means of goaldirected movements of a trunkpositioned pharynx, following protrusion of the pharynx out of the body, raising the question of how planarians control such a complex set of body movements for achieving robust feeding.
Transcriptome profiling and digital gene expression by deep. Dugesia japonica is a branch of planarian organism. Unigene sequences are first aligned by blastx to protein databases such as nr. Use the browse button to upload a file from your local disk. Interestingly, in the process of regeneration, there is little wound infection, suggesting that d. Molecular phylogenetics and sequence analysis of two cave. Original research the effect of perfluorooctanoic acid on. To facilitate adoption of this model and implementation of such methods, we showcase in this video article protocols for pharmacological and genetic assays in vivo rnai by feeding using the planarian dugesia japonica. The pharyngeal nervous system orchestrates feeding. Regeneration of the epidermis and basement membrane of the. Comprehensive gene expression analyses in pluripotent stem. Fatsia japonica fatsia page 3 october 1999 figure 3. Dugesia japonica is the best suited of three planarian. Expression and functional analysis of flotillins in.
However, the roles of flotillins in dugesia japonica have not been addressed yet, the aim of this study is to reveal the functions of flotillins during planarians regeneration, and provides basic data for the. Comparative and transcriptome analyses uncover key aspects. Morphological changes of gonad and gene expression. Djrlc is required for the intestinal regeneration in. The data may be either a list of database accession numbers, ncbi gi numbers, or sequences in fasta format. Analysis of ploidy in a planarian by flow cytometry. Researchers induce planarian flatworms to grow heads, brains. Research we are currently interested in three main research areas.
Usually they have grey, brown or black colors on the dorsal body surface, the ventral surface uses to be paler. Cloning and characterization of djprps gene in freshwater. However, there are some important differences between s. To clone the phosphoribosyl pyrophosphate synthetase gene from dugesia japonica, and to do the bioinformatics and spatial expression analysis of the gene. Groups interested in participating in the linkout program should visit the linkout home page. The schmidtea mediterranea database as a molecular. The germination database covers germination information for a particular plant. Cloning and characterization of djprps gene in freshwater planarian dugesia japonica djprps geninin tatl. The bacterial metabolite indole inhibits regeneration of. Dugesiidae zimei dong 1, changying shi 1, gengbo chu 1, yanping dong.
Expression and functional analysis of flotillins in dugesia. In the present study, we isolate three planarian dugesia japonica atg8 genes djatg81, djatg82, djatg83 that show high sequence similarity with atg8 from. Wholegenome sequences of bacteria isolated from the dugesia japonica microbiome have been deposited in ncbi as qaji00000000qajs00000000 see table s2. Itis taxonomy is based on the latest scientific consensus available, and is provided as a general reference source for interested parties. Hosted by the usgs core science analytics and synthesis. Because it is under the genus dugesia, it is also sometimes considered a type of planarian phylogeny.
Planarians are nonparasitic platyhelminthes flatworms famous for their regeneration ability and for having a wellorganized brain. This figure shows gene alignment by mauve software, and indicates the complexity of three structures djpiwib genes. Exploiting endogenous ion transport for regenerative therapies will require direct regulation of membrane voltage. Jan 17, 2020 each species has a set of predefined gene expression patterns corresponding to each organ in the anatomy ontology. The planarian djpreb cdna is comprised of 1101 bp and contains a 972 bp open reading frame corresponding to a deduced protein of 323 amino acids with a 69 bp 5utr and a 60 bp 3utr. The planarians dugesia japonica are distributed widely in china, japan, korea, and southern siberia.
Micrornas mirnas, which are 18 24 nucleotides length, play important roles in regulating the expression of gene at the posttranscription level. Here we conducted largescale est analysis of the head region of the planarian dugesia japonica to construct a database of the headregion transcriptome, and then performed comparative analyses among related species. Dugesia species have an elongated body with a slightly triangleshaped head. In this study, we report the expression and identification of a prebrelated gene from the planarian dugesia japonica, djpreb. Flow cytometry flow microfluorimetry provides a quick means for analysis of ploidy in planarians. Because it is under the genus dugesia, it is also sometimes considered a type of planarian. The plant in garden database relates the plants to a particular garden. Here we conducted largescale est analysis of the head region of the planarian dugesia japonica to construct a database of the headregion transcriptome. Collins a, c, a department of biology, swarthmore college, swarthmore, pa, usa. Blastp simply compares a protein query to a protein database.
Dugesia japonica is the best suited of three planarian species for highthroughput toxicology screening danielle ireland a, veronica bochenek a, daniel chaiken b, christina rabeler a, sumi onoe b, ameet soni b, evamaria s. Page designed through the cooperative efforts of interagency itis teams. How planarians reproduce by ripping themselves into a head and a tail piece, which subsequently regenerate into two new worms, is a centuriesold biomechanics problem. A recent study has shown that arg65, phe104 and tyr106 in yeast atg8 are highly conserved residues that are essential for the conjugation. The bacterial metabolite indole inhibits regeneration of the. We must overcome these problems to provide useful databases for. Chan jd, zhang d, liu x, zarowiecki mz, berriman m, marchant js. Curation and annotation of planarian gene expression patterns.
Tissues from sexually mature individuals produced histograms with a. Michael faraday contemplated how this feat can be achieved in the 1800s, but it remained unanswered because it is experimentally difficult to observe planarians doing it. Pdf molecular phylogenetics and sequence analysis of two. Dugesia japonica is a common planarian organism in east asia. Molecular cloning and expression pattern of the djstag gene. Here we conducted largescale est analysis of the head region of the planarian dugesia japonica to construct a database of the headregion transcriptome, and then performed comparative analyses. Pdf comparative transcriptome analysis between planarian. As an integral component of tissue homeostasis and regeneration, remodeling occurs constantly in the general planarian life history. Dugesia ryukyuenis and dugesia japonica sakai and sakaizumi. This data article provides a transcriptomic resource for the free living planarian flatworm dugesia japonica related to the research article entitled utilizing the planarian voltagegated ion channel. Biophysical signaling is required for both embryonic polarity and regenerative outgrowth. Material and methods animals and egg capsules the planarians and egg capsules used belong to the race of d. Produce classroom dramawatch and study regenerationsave moneyfor a class of 30 students. Dugesia is a member of the class turbellaria and is freeliving.
The complete mitochondrial genome of dugesia japonica. Planarians are the earliest freeliving platyhelminthe with triploblastic and bilateralsymmetry. Rnai of cnsexpressed gene djslk induces morphogenetic malformation and death in planarian dugesia japonica x chen1,2, h zhen1, s wu1, q lu1, q pang1, b zhao1 1laboratory of developmental and evolutionary biology, school of life sciences, shandong university of technology, zibo 255049, p. Djpiwib djpiwib dugesia japonica planarian djpiwib gene. To clone the phosphoribosyl pyrophosphate synthetase gene from dugesia japonica. Faculty collaboration database dataset for a dugesia. Comprehensive gene expression analyses in pluripotent stem cells of a planarian, dugesia japonica norito shibata,1, tetsutaro hayashi2, ryutaro fukumura3, junsuke fujii4, tomomi. Candidatus trichorickettsia mobilis, a rickettsiales. Complete template that allows you to organize and manage all information about your favorite plants. Planarians are members of the phylum platyhelminthes, a. Nuclei from homogenized tissues of the freshwater planarian dugesia japonica japonica ichikawa et kawakatsu were stained with propidium iodide and measured with an argonlaser flow cytometer to produce histograms of dna content. A total of 54,752 highquality est reads were obtained from a head library of the planarian dugesia. Dugesia japonica possesses a putative atg8 protein. It has emerged as a powerful model system for studying the mechanism of development and regeneration newmark and alvarado, 2002.
Autophagyrelated djatg8 is required for remodeling in. However, the roles of flotillins in dugesia japonica have not been addressed yet, the aim of this study is to reveal the functions of flotillins during planarians regeneration, and provides basic data for the functions of flotillins and the molecular mechanism of planarian regeneration. The garden database lists gardens, their gardeners and the speciality of that garden. Oct 01, 2012 we used two sequencing methods, namely long polymerase chain reaction pcr and primer walking, to determine the complete mitochondrial dna mtdna sequence of dugesia japonica and most of the mtdna sequence of dugesia ryukyuensis. The protein blast software identifies the conserved regions and the. Planarians are inhabitants of cool, clear and permanent water and cling to the undersurfaces of leaves, logs, rocks sub merged in streams, ponds and lakes. Several planarian species, such as dugesia japonica and schmidtea. Dugesia japonica is a species of dugesiid triclad that inhabits the freshwater bodies of east asia.
Morphological changes of gonad and gene expression patterns during desexualization in dugesia japonica platyhelminthes. Research article open access comparative transcriptome. However, it is not a legal authority for statutory or. Dugesia japonica is a typical planarian species that is widely distributed in the. The ncbi taxonomy database allows browsing of the taxonomy tree, which contains a. Article the bacterial metabolite indole inhibits regeneration of the planarian flatworm dugesia japonica fredrick j. Frans plants database free download tucows downloads. Blastx search protein databases using a translated nucleotide query. Psiblast allows the user to build a pssm positionspecific scoring matrix using the results of the first blastp run. Smedob1 is required for planarian homeostasis and regeneration. Planarians used in this work belonged to the species dugesia japonica, asexual strain gi rossi et al. Molecular phylogenetics and sequence analysis of two cavedwelling dugesia species from southeast asia platyhelminthes. The speciose freshwater planarian genus dugesia girard, 1850 has been extensively studied for more.
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